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Urine Culture Contamination among Infants

Author: Suhana Hashim

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Abstract

Suhana Hashim1, Aliyaisma Ariffin2, Noor Hafizan Mat Salleh3

Objectives

Urine culture & sensitivity (C&S) is an important test to support the diagnosis of urinary tract infection.  However, it is easily contaminated during handling the specimen. A contaminated urine culture is  defined as the presence of more than 1 isolation at greater than or equal to 100 000 CFU/mL. 40% is  the standard contamination rate for this study. The aim of this study is to reduce the contamination rate  of urine culture among infants in the health clinics under Kota Bharu district, to identify the contributing  factors and to provide strategic intervention to overcome the problem.

Methods

A cross-sectional study comparing pre and post interventions was conducted in 2019. Data were  collected from the questionnaires, laboratory information system (LIS) named Sistem Informasi  Makmal Kesihatan Awam (SIMKA) and urine C&S lab form. A total of 40 validated questionnaires  were given to the clinic staffs randomly at 10 health clinics. Data were analysed and intervention were  carried out to tackle the problem. CME on proper procedure in urine C&S and the practical session on  Bladder Stimulation Technique as alternative way in clean catch urine method were done at selected  health clinic. CDs, posters, and flyers on standard procedure of urine C&S also had been distributed to  all health clinic in Kelantan.

Results

Post intervention, the urine contamination rate slightly reduced from 59.6% to 52.5% which is still  higher than the standard (<40%). In general, the percentage of the good practice increasing for urine  collection, storage and transportation. The level of knowledge for category ‘poor’ was reducing from  5% to zero. Clean catch urine as the preference method for the collection of urine also increasing from  17.5% to 32.5%.

Conclusion

Continuous monitoring of urine culture contamination and sustaining the remedial measures are very  important to control the problem. There is room for improvement to reduce the contamination rate of  urine culture among infant for sample received in Kota Bharu Public Health Laboratory.

 

Keywords: Urine Culture Contamination, Urine C&S, Infants, Clean Catch Urine

  1. Dr Suhana Hashim, Clinical Microbiologist, Kota Bharu Public Health Laboratory, Lot 522 KM 10 Jalan Kuala Krai, 16010 Kota Bharu Kelantan, Malaysia.
  2. Science Officer, Kota Bharu Public Health Laboratory.
  3. Public Health Specialist, Director of Kota Bharu Public Health Laboratory.

Correspondence to:

Dr Suhana Hashim, Clinical Microbiologist,

Kota Bharu Public Health Laboratory,

Lot 522 KM 10 Jalan Kuala Krai,

16010 Kota Bharu Kelantan, Malaysia,

Email: [email protected]

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